Wild-type (WT) and alpha Glucosidase II knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Glucosidase II antibody [C2C3], C-term (GTX102205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded SG xenograft , using alpha Glucosidase II(GTX102205) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (30 ug of whole cell lysate)
A: Hela
B: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX102205 diluted at 1:1000