Various tissue extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX133356) diluted at 1:3000.The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

alpha Internexin antibody detects alpha Internexin protein at neurofilament in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
alpha Internexin antibody (GTX133356) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Internexin antibody detects alpha Internexin protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: alpha Internexin protein stained by alpha Internexin antibody (GTX133356) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

alpha Internexin antibody detects alpha Internexin protein at neurofilament in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
alpha Internexin antibody (GTX133356) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Internexin antibody detects alpha Internexin protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Internexin protein stained by alpha Internexin antibody (GTX133356) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).