Immunofluorescence analysis of methanol-fixed A431, using alpha SNAP(GTX106626) antibody at 1:200 dilution.

alpha SNAP antibody [N2C3] detects alpha SNAP protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
alpha SNAP antibody [N2C3] (GTX106626) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

alpha SNAP antibody [N2C3] detects alpha SNAP protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha SNAP protein stained by alpha SNAP antibody [N2C3] (GTX106626) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with alpha SNAP antibody [N2C3] (GTX106626) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
10% SDS PAGE
GTX106626 diluted at 1:1000

The data was published in the journal Evid Based Complement Alternat Med in 2013.PMID: 23573126