Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Skeletal Muscle Actin antibody (GTX101362) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Skeletal Muscle Actin antibody (GTX101362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
10% SDS PAGE
GTX101362 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
D: C2C12
10% SDS PAGE
GTX101362 diluted at 1:5000

Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using alpha Actin (skeletal muscle)(GTX101362) antibody(10 μg/ml).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min