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alpha Smooth Muscle Actin antibody [HL3088]

Recombinant antibody Orthogonal
Anti-alpha Smooth Muscle Actin antibody [HL3088] used in Western Blot (WB). GTX640534
Anti-alpha Smooth Muscle Actin antibody [HL3088] used in Western Blot (WB). GTX640534

Cat. No. GTX640534

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL3088

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all alpha Smooth Muscle Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of human alpha Smooth Muscle Actin.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin alpha 2, smooth muscle , ACTSA

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017]

Database

  • Gene ID: 59 ACTA2
  • UniProt: P62736 ACTA2

Research Area

DATA IMAGES

Anti-alpha Smooth Muscle Actin antibody [HL3088] used in Western Blot (WB). GTX640534

GTX640534 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody [HL3088] (GTX640534) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-alpha Smooth Muscle Actin antibody [HL3088] used in Western Blot (WB). GTX640534

GTX640534 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody [HL3088] (GTX640534) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Available soon.
Custom Conjugations