Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein at cytoplasm on human liver carcinoma stroma by immunohistochemical analysis.
Sample: Paraffin-embedded liver carcinoma stroma.
alpha smooth muscle Actin antibody (GTX100034) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

alpha smooth muscle Actin antibody detects ACTA2 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
alpha smooth muscle Actin antibody (GTX100034) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha smooth muscle Actin antibody (GTX100034) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cervix.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human normal colon tissue.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, 20 min

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cervix.
Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Biomolecules in 2019. PMID: 31443530

The data was published in the 2022 in J Biomed Sci. PMID: 35765067

The data was published in the journal PLoS One in 2019. PMID: 30695033

The data was published in the journal Front Pharmacol in 2016. PMID: 27199755