Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Tubulin(GTX102079) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
10% SDS PAGE
GTX102079 diluted at 1:10000

alpha Tubulin antibody detects TUBA1B protein at cytoskeleton by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TUBA1B protein stained by alpha Tubulin antibody (GTX102079) diluted at 1:500.
Blue: Hoechst 33342 staining.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX102079) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

alpha Tubulin antibody detects alpha Tubulin protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
alpha Tubulin antibody (GTX102079) dilution: 1:200.

The data was published in the journal PLoS One in 2017. PMID: 28301550