Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using alpha Tubulin(GTX110717) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX110717) diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Tubulin(GTX110717) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX110717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using alpha Tubulin antibody (GTX110717) at 1:300 dilution.