GeneTex
  • Country / Location Selection

United States (US)

alpha Tubulin antibody

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX110717
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX110717
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110717
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX110717
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX110717

Cat. No. GTX110717

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724856

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

K-ALPHA-1 antibody , TUBA1B antibody , alpha-tubulin ubiquitous antibody , alpha tubulin antibody , tubulin K-alpha-1 antibody , tubulin alpha-ubiquitous chain antibody , "tubulin , alpha , ubiquitous antibody" , tubulin alpha-1B chain antibody , "tubulin , alpha 1b antibody"

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX110717

GTX110717 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Tubulin(GTX110717) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX110717

GTX110717 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX110717) diluted at 1:1000.

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110717

GTX110717 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using alpha Tubulin(GTX110717) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX110717

GTX110717 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using alpha Tubulin antibody (GTX110717) at 1:300 dilution.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX110717

GTX110717 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX110717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for alpha Tubulin antibody (GTX110717). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for alpha Tubulin antibody (GTX110717). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169