Non-transfected (–) and transfected (+) unboiled MDA-MB-231 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta 1 Adrenergic Receptor antibody [HL3678] (GTX641706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.