Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

beta 2 Microglobulin antibody immunoprecipitates B2M protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of B2M protein by 4 μg beta 2 Microglobulin antibody (GTX112815)
15 % SDS-PAGE
The immunoprecipitated B2M protein was detected by beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

beta 2 Microglobulin antibody detects beta 2 Microglobulin protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta 2 Microglobulin stained by beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Beta 2 Microglobulin antibody detects secreted B2M protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
B2M stained by Beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

beta 2 Microglobulin antibody detects beta 2 Microglobulin protein at cytoplasm in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse plasma (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.

beta 2 Microglobulin antibody detects B2M protein at cytosol on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
beta 2 Microglobulin antibody (GTX112815) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min