Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Beta-crystallin A4(GTX109526) antibody at 1:200 dilution.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with beta A4 Crystallin antibody [N1C3-2] (GTX109526) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with beta A4 Crystallin antibody [N1C3-2] (GTX109526) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX109526 diluted at 1:1000