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beta Actin antibody

KO/KD Comparison
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110564
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Immunoprecipitation (IP). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564
Anti-beta Actin antibody used in Western Blot (WB). GTX110564

Cat. No. GTX110564

Reference 42

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, IHC

Reactivity

 Human, Mouse, Rat, Yeast, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all beta Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

rat liver , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618080

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

beta Actin antibody detects beta Actin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with beta Actin antibody (GTX110564) diluted at 1:10000 and competitor's antibody (sc-130656) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

beta Actin antibody detects ACTB protein by western blot analysis.
A. 30 μg drosophila lysate/extract
10% SDS-PAGE
beta Actin antibody (GTX110564) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX110564

GTX110564 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
beta Actin antibody (GTX110564) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

beta Actin antibody detects beta Actin protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110564

GTX110564 ICC/IF Image

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.
Green: beta Actin protein stained by beta Actin antibody (GTX110564) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX110564

GTX110564 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
beta Actin antibody (GTX110564) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Immunoprecipitation (IP). GTX110564

GTX110564 IP Image

Immunoprecipitation of beta Actin protein from 293T whole cell extracts using 5 μg of beta Actin antibody (GTX110564).
Western blot analysis was performed using beta Actin antibody (GTX110564).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

Yeast lysate (10 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX110564

GTX110564 IHC-P Image

beta Actin antibody detects beta Actin protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
beta Actin antibody (GTX110564) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

The data was published in the journal Front Physiol in 2017.PMID: 28588500

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

The data was published in the journal PLoS One in 2016.PMID: 27486856

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

The data was published in the journal Aging Cell in 2017. PMID: 27623715

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

The data was published in the journal Aging Cell in 2017. PMID: 27623715

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

The data was published in the journal Aging Cell in 2017. PMID: 27623715

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

The data was published in the journal PLoS Pathog in 2019. PMID: 30818369

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

The data was published in the journal PLoS Pathog in 2019. PMID: 30818369

Anti-beta Actin antibody used in Western Blot (WB). GTX110564

GTX110564 WB Image

The data was published in the journal Redox Biol in 2014. PMID: 24936442

REFERENCE

Application Reference

Wu Z et al. Biochem Biophys Rep 2024; 38 : 101680 TLR2-dependent and independent pyroptosis in dTHP-1 cells induced by Actinomyces oris MG-1.
Shimada M et al. J Radiat Res 2023; 64 (2) : 345-351 Implication of E3 ligase RAD18 in UV-induced mutagenesis in human induced pluripotent stem cells and neuronal progenitor cells.
Young MJ et al. J Biomed Sci 2022; 29 (1) : 3 Estradiol-mediated inhibition of Sp1 decreases miR-3194-5p expression to enhance CD44 expression during lung cancer progression.
Application : WB
Reactivity : Human
Ting-Yu Chao et al. Food Sci Nutr 2021; 9 (9) : 4758-4769 Bracteanolide A abrogates oxidative stress-induced cellular damage and protects against hepatic ischemia and reperfusion injury in rats.
Application : WB
Reactivity : Human
Dai J et al. Elife 2021; 10 Curcumin promotes AApoAII amyloidosis and peroxisome proliferation in mice by activating the PPARα signaling pathway.
Application : WB
Reactivity : Mouse
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REVIEW

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Application Reference
Wu Z et al. Biochem Biophys Rep 2024; 38:101680 TLR2-dependent and independent pyroptosis in dTHP-1 cells induced by Actinomyces oris MG-1.
Shimada M et al. J Radiat Res 2023; 64 (2):345-351 Implication of E3 ligase RAD18 in UV-induced mutagenesis in human induced pluripotent stem cells and neuronal progenitor cells.
Young MJ et al. J Biomed Sci 2022; 29 (1):3 Estradiol-mediated inhibition of Sp1 decreases miR-3194-5p expression to enhance CD44 expression during lung cancer progression.
Application : WB
Reactivity : Human
Ting-Yu Chao et al. Food Sci Nutr 2021; 9 (9):4758-4769 Bracteanolide A abrogates oxidative stress-induced cellular damage and protects against hepatic ischemia and reperfusion injury in rats.
Application : WB
Reactivity : Human
Dai J et al. Elife 2021; 10 Curcumin promotes AApoAII amyloidosis and peroxisome proliferation in mice by activating the PPARα signaling pathway.
Application : WB
Reactivity : Mouse
Brahma MK et al. J Am Heart Assoc 2020; 9 (15):e013039 Increased Glucose Availability Attenuates Myocardial Ketone Body Utilization.
Application : WB
Reactivity : Mouse
Wang SA et al. Sci Rep 2020; 10 (1):20870 USP24 stabilizes bromodomain containing proteins to promote lung cancer malignancy.
Application : WB
Reactivity : Human
Satou R et al. Heliyon 2019; 5 (11):e02792 Light conditions affect rhythmic expression of aquaporin 5 and anoctamin 1 in rat submandibular glands.
Khanal S et al. PLoS Pathog 2019; 15 (2):e1007442 High-risk human papillomavirus oncogenes disrupt the Fanconi anemia DNA repair pathway by impairing localization and de-ubiquitination of FancD2.
Ryouichi Satou et al. Heliyon 2019; (Epub) Light conditions affect rhythmic expression of aquaporin 5 and anoctamin 1 in rat submandibular glands.
Application : WB
Reactivity : Rat
Yi-Chang Wang et al. Nature Communication 2018; 9:3996 USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy.
Application : WB
Reactivity : Human
Nashine S et al. Sci Rep 2018; 8 (1):15175 Characterizing the protective effects of SHLP2, a mitochondrial-derived peptide, in macular degeneration.
Application : WB
Reactivity : Human
Gong W et al. J Cell Biochem 2018; (Epub) Deletion of long noncoding RNA EFNA3 aggravates hypoxia-induced injury in PC-12 cells by upregulation of miR-101a.
Application : WB
Reactivity : Rat
Weng TY et al. Sci Rep 2018; 8 (1):8216 Homoharringtonine induced immune alteration for an Efficient Anti-tumor Response in Mouse Models of Non-small Cell Lung Adenocarcinoma Expressing Kras Mutation.
Application : WB
Reactivity : Human
Satou R et al. Front Physiol 2017; 8:320 Temporal Expression Patterns of Clock Genes and Aquaporin 5/Anoctamin 1 in Rat Submandibular Gland Cells.
Application : WB
Reactivity : Rat
Shimada T et al. Biomed Res 2017; 38 (5):269-276 CCL5 is induced by TLR 3 signaling in HuCCT1 human biliary epithelial cells: possible involvement in the pathogenesis of biliary atresia.
Application : WB
Reactivity : Human
Kolanowska M et al. Sci Rep 2017; 7 (1):9942 Functional analysis of a novel, thyroglobulin-embedded microRNA gene deregulated in papillary thyroid carcinoma.
Application : WB
Reactivity : Human
Lao S et al. Mol Med Rep 2017; 16 (1):507-514 A comparative study of the influence of two types of PHEMA stents on the differentiation of ASCs to myocardial cells.
Application : WB
Reactivity : Human
Wang YY et al. Cancer Chemother Pharmacol 2017; 79 (6):1129-1140 CYT-Rx20 inhibits ovarian cancer cells in vitro and in vivo through oxidative stress-induced DNA damage and cell apoptosis.
Application : WB
Reactivity : Human
Wang YY et al. Head Neck 2017; (Epub) Synthetic β-nitrostyrene derivative CYT-Rx20 as inhibitor of oral cancer cell proliferation and tumor growth through glutathione suppression and reactive oxygen species induction.
Application : WB
Reactivity : Human
Shirai K et al. Brain Res 2017; 1658:60-67 * (ifn)-induced protein 35 (ifi35) negatively regulates ifn-β-phosphorylated stat1-rig-i-cxcl10/ccl5 axis in u373mg astrocytoma cells treated with polyinosinic-polycytidylic acid.
Hsu YC et al. Oncotarget 2016; 7 (52):86239-86256 Discovery of BPR1K871, a quinazoline based, multi-kinase inhibitor for the treatment of AML and solid tumors: Rational design, synthesis, in vitro and in vivo evaluation.
Nashine S et al. PLoS One 2016; 11 (8):e0159828 Differential Expression of Complement Markers in Normal and AMD Transmitochondrial Cybrids.
Application : WB
Reactivity : Human
Liao HF et al. Sci Rep 2016; 6:27350 Down-regulated and Commonly mutated ALPK1 in Lung and Colorectal Cancers.
Application : ICC/IF
Reactivity : Human
Ishii T et al. Aging Cell 2016; Endogenous reactive oxygen species cause astrocyte defects and neuronal dysfunctions in the hippocampus: a new model for aging brain.
Application : WB
Reactivity : Mouse
Chen YT et al. Oncotarget 2016; (Epub) Inhibition of DNA methyltransferase 1 increases nuclear receptor subfamily 4 group A member 1 expression and decreases blood glucose in type 2 diabetes.
Application : WB
Reactivity : Human
Czajka AA et al. PLoS One 2016; 11 (3):e0151968 Family of microRNA-146 Regulates RARβ in Papillary Thyroid Carcinoma.
Application : IHC
Reactivity : Human
Graham MK et al. Biochemistry 2015; 54 (13):2270-82 Targeting the human androgen receptor gene with platinated triplex-forming oligonucleotides.
Application : WB
Reactivity : Human
Chen YT et al. Oncotarget 2015; 6 (15):12997-3005 ptprd silencing by dna hypermethylation decreases * receptor signaling and leads to type 2 diabetes.
Application : WB
Reactivity : Human
Chen HY et al. Oncogene 2015; KLHL39 suppresses colon cancer metastasis by blocking KLHL20-mediated PML and DAPK ubiquitination.
Application : WB
Reactivity : Human
Lin GJ et al. Toxicol Appl Pharmacol 2015; 282 (2):207-14 Dimethyl sulfoxide inhibits spontaneous diabetes and autoimmune recurrence in non-obese diabetic mice by inducing differentiation of regulatory T cells.
Application : WB
Reactivity : Mouse
Ishii T et al. Redox Biol 2014; 2:679-85 Genetically induced oxidative stress in mice causes thrombocytosis, splenomegaly and placental angiodysplasia that leads to recurrent abortion.
Liu C et al. J Neurosci 2014; 34 (37):12289-303 The Active Zone Protein Family ELKS Supports Ca2+ Influx at Nerve Terminals of Inhibitory Hippocampal Neurons.
Application : WB
Reactivity : Mouse
Her LS et al. Brain Pathol 2014; The differential profiling of ubiquitin-proteasome and autophagy systems in different tissues before the onset of Huntington's disease models.
Application : WB
Reactivity : Mouse
Wei MF et al. Autophagy 2014; 10 (7):1179-92 Autophagy promotes resistance to photodynamic therapy-induced apoptosis selectively in colorectal cancer stem-like cells
Application : WB
Reactivity : Human
Luo CW et al. Biochim Biophys Acta 2014; 1843 (9):2129-2137 Radiation sensitization of tumor cells induced by shear stress: The roles of integrins and FAK.
Application : WB
Reactivity : Human
Ishii T et al. Redox Biology 2014; 2:679-685 Genetically induced oxidative stress in mice causes thrombocytosis, splenomegaly and placental angiodysplasia that leads to recurrent abortion
Application : WB
Reactivity : Mouse
Zhang G et al. PLoS One 2014; 9 (4):e95872 Enhanced antitumor efficacy of an oncolytic herpes simplex virus expressing an endostatin-angiostatin fusion gene in human glioblastoma stem cell xenografts.
Application : WB
Reactivity : Human
Lin MC et al. Oral Oncol 2014; GALNT2 enhances migration and invasion of oral squamous cell carcinoma by regulating EGFR glycosylation and activity.
Application : WB
Reactivity : Human
Lin WH et al. PLoS One 2014; 9 (1):e83160 Evaluation of the antitumor effects of BPR1J-340, a potent and selective FLT3 inhibitor, alone or in combination with an HDAC inhibitor, vorinostat, in AML cancer.
Application : WB
Reactivity : Human
Hung CJ et al. J Endod 2013; 39 (12):1557-61 Antiosteoclastogenic activity of silicate-based materials antagonizing receptor activator for nuclear factor kappaB ligand-induced osteoclast differentiation of murine marcophages.
Application : WB
Reactivity : Mouse
Chen WT et al. PLoS One 2012; 7 (4):e35807 Amyloid-beta (Aβ) D7H mutation increases oligomeric Aβ42 and alters properties of Aβ-zinc/copper assemblies.
Application : WB
Reactivity : Human
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