Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

beta Tubulin 3/ Tuj1 antibody [HL1709] detects beta Tubulin 3/ Tuj1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

beta Tubulin 3/ Tuj1 antibody [HL1709] detects beta Tubulin 3/ Tuj1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

beta Tubulin 3/ Tuj1 antibody [HL1709] detects beta Tubulin 3/ Tuj1 protein by immunofluorescent analysis.
Sample: SH-SY-5Y cells were fixed in ice-cold MeOH for 10 min.
Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

beta Tubulin 3/ Tuj1 antibody [HL1709] detects beta Tubulin 3/ Tuj1 protein at cytoskeleton by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.