Sample (30 ug of whole cell lysate) 
      A: NIH-3T3
      B: JC
C: BCL-1
      D: C2C12
      E: Raw264.7
      10% SDS PAGE 
      GTX112659 diluted at 1:1000

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
10% SDS PAGE
GTX112659 diluted at 1:10000

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using beta Tubulin(GTX112659) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX112659 diluted at 1:1000

Immunofluorescence analysis of methanol-fixed HeLa, using beta Tubulin(GTX112659) antibody at 1:500 dilution.