WB (peptide competition) analysis of fibroblasts transfected with oncogenic DSH3-Abl using GTX24479 c-Abl (phospho Tyr245) antibody with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

ICC/IF analysis of HeLa cells treated with 4 uM Curcumin for 4hr using GTX24479 c-Abl (phospho Tyr245) antibody. Panel e shows the untreated cells with nuclear localization. Panel e shows the no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes