ICC/IF analysis of HeLa cells were treated with PBS (A) or stimulated with 20% FBS (B) for 30 min after serum stravation for 36hr using GTX03375 c-Fos antibody. c-FOS antibody labels only the nuclei of stimulated cells.
Red : Primary antibody
Green : Tubulin
Blue : DAPI
Dilution : 1:2000

IHC (Free Floating) analysis of Mouse hippocampus (C) or olfactory bulb (D) tissue sections using GTX03375 c-Fos antibody. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.
Red : Primary antibody
Green : NF-L
Blue : Hoechst staining of nuclear DNA
Dilution : 1:10000

WB analysis of various sample lysates using GTX03375 c-Fos antibody. Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein.
Red : Primary antibody
Green : GAPDH
Dilution : 1:2000
Lane 1 : protein marker
Lane 2 : HeLa cells grown in FBS free media
Lane 3 : HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours
Lane 4 : rat cortical neurons
Lane 5 : rat cortical neurons treated with membrane depolarization buffer for 5 hours