Specificity test was performed by coating the plate with MET peptide, MET (phospho Tyr1234) peptide, MET (phospho Tyr1235) peptide, and MET (phospho Tyr1234/Tyr1235) peptide (15.63 ng/mL). Coated peptides were probed with c-Met (phospho Tyr1234) antibody [HL2907] (GTX640244) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met (phospho Tyr1234) antibody [HL2907] (GTX640244) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.