Wild-type (WT) and c-Myc knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody [N1], N-term (GTX109636) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

c-Myc antibody [N1], N-term detects c-Myc protein by Western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Myc antibody [N1], N-term (GTX109636) diluted at 1:1000.