Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:1000.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:500.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:500.

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
7.5% SDS PAGE
GTX102287 diluted at 1:1500

eEF2 antibody detects eEF2 protein at cytosol on human colon by immunohistochemical analysis.
Sample: Paraffin-embedded colon.
eEF2 antibody (GTX102287) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

eEF2 antibody detects eEF2 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
eEF2 antibody (GTX102287) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX102287 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using eEF2 antibody [N2C1], Internal (GTX102287) at 1:300 dilution.