WB analysis of whole cell extracts (20 μg lysate) of COS-7 (Lane 1), MCF7 (Lane 2), PC-12 (Lane 3), HeLa (lane 4), Jurkat (lane 5), NIH/3T3 (lane 6) and A-431 (lane 7) using GTX25369 eIF2 alpha antibody [EIF2a].
Dilution : 1:500-1:1000

IHC-P analysis of human colon carcinoma tissue using GTX25369 eIF2 alpha antibody [EIF2a].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

ICC/IF analysis of A549 cells using GTX25369 eIF2 alpha antibody [EIF2a]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in1% BSA and incubated for 3 hours at room temperature

IHC-P analysis of human colon carcinoma tissue using GTX25369 eIF2 alpha antibody [EIF2a].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20