Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with eIF2 alpha antibody [N1C3] (GTX101241) diluted at 1:5000.

eIF2 alpha antibody [N1C3] detects eIF2 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eIF2 alpha protein stained by eIF2 alpha antibody [N1C3] (GTX101241) diluted at 1:5000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

eIF2 alpha antibody [N1C3] detects eIF2 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eIF2 alpha protein stained by eIF2 alpha antibody [N1C3] (GTX101241) diluted at 1:500.
Blue: Hoechst 33342 staining.