293T whole cell and cytoplasm extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1553] (GTX637028) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

eIF4E antibody [HL1553] detects eIF4E protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
eIF4E stained by eIF4E antibody [HL1553] (GTX637028) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1553] (GTX637028) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

eIF4E antibody [HL1553] detects eIF4E protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
eIF4E stained by eIF4E antibody [HL1553] (GTX637028) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 8.0, 15 min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1553] (GTX637028) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1553] (GTX637028) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

eIF4E antibody [HL1553] detects eIF4E protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: eIF4E stained by eIF4E antibody [HL1553] (GTX637028) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).