Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with gamma C Crystallin antibody (GTX103004) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
12% SDS PAGE
GTX103004 diluted at 1:1000