Wild-type (WT) and hnRNP A2B1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX114475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX114475) diluted at 1:2000.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using hnRNP A2B1(GTX114475) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX114475 diluted at 1:5000

hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP A2B1 protein stained by hnRNP A2B1 antibody (GTX114475) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.