Wild-type (WT) and hnRNP R knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with hnRNP R antibody [N1N2], N-term (GTX106526) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
7.5% SDS PAGE
GTX106526 diluted at 1:3000

hnRNP R antibody [N1N2], N-term detects hnRNP R protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP R protein stained by hnRNP R antibody [N1N2], N-term (GTX106526) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.