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mGluR5 antibody [HL2543]

Recombinant antibody Orthogonal
Anti-mGluR5 antibody [HL2543] used in Western Blot (WB). GTX638912
Anti-mGluR5 antibody [HL2543] used in IHC (Paraffin sections) (IHC-P). GTX638912
Anti-mGluR5 antibody [HL2543] used in IHC (Paraffin sections) (IHC-P). GTX638912
Anti-mGluR5 antibody [HL2543] used in IHC (Paraffin sections) (IHC-P). GTX638912
Anti-mGluR5 antibody [HL2543] used in IHC (Paraffin sections) (IHC-P). GTX638912
Anti-mGluR5 antibody [HL2543] used in Western Blot (WB). GTX638912

Cat. No. GTX638912

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2543

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all mGluR5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

Product Note

This antibody was raised against human mGluR5 Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human mGluR5. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate metabotropic receptor 5 , GPRC1E , MGLUR5 , PPP1R86 , mGlu5

Cellular Localization

Cell membrane

Background

This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-mGluR5 antibody [HL2543] used in Western Blot (WB). GTX638912

GTX638912 WB Image

Orthogonal

Boiled and unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL2543] (GTX638912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-mGluR5 antibody [HL2543] used in IHC (Paraffin sections) (IHC-P). GTX638912

GTX638912 IHC-P Image

mGluR5 antibody [HL2543] detects mGluR5 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
mGluR5 stained by mGluR5 antibody [HL2543] (GTX638912) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-mGluR5 antibody [HL2543] used in IHC (Paraffin sections) (IHC-P). GTX638912

GTX638912 IHC-P Image

mGluR5 antibody [HL2543] detects mGluR5 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
mGluR5 stained by mGluR5 antibody [HL2543] (GTX638912) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-mGluR5 antibody [HL2543] used in IHC (Paraffin sections) (IHC-P). GTX638912

GTX638912 IHC-P Image

mGluR5 antibody [HL2543] detects mGluR5 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
mGluR5 stained by mGluR5 antibody [HL2543] (GTX638912) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-mGluR5 antibody [HL2543] used in IHC (Paraffin sections) (IHC-P). GTX638912

GTX638912 IHC-P Image

mGluR5 antibody [HL2543] detects mGluR5 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
mGluR5 stained by mGluR5 antibody [HL2543] (GTX638912) diluted at 1:300.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-mGluR5 antibody [HL2543] used in Western Blot (WB). GTX638912

GTX638912 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL2543] (GTX638912) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations