Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3433] (GTX641275) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3433] (GTX641275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled rat tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mGluR5 antibody [HL3433] (GTX641275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.