Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2217] (GTX638221) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of mTOR protein from HeLa whole cell extract using 5 μg of mTOR antibody [HL2217] (GTX638221).
Western blot analysis was performed using mTOR antibody [HL2217] (GTX638221).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

mTOR antibody [HL2217] detects mTOR protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
mTOR stained by mTOR antibody [HL2217] (GTX638221) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2217] (GTX638221) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2217] (GTX638221) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2217] (GTX638221) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.