Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mtRNA polymerase antibody [N2N3] (GTX105137) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed Hep G2, using mtRNA polymerase(GTX105137) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded human hepatoma, using mtRNA polymerase(GTX105137) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
5% SDS PAGE
GTX105137 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the 2013 in Nucleic Acids Res. PMID: 23982517