mtTFA antibody immunoprecipitates mtTFA protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 μg of mtTFA antibody (GTX112760) 12% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody (GTX112760) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:1000.

mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.
Green: mtTFA protein stained by mtTFA antibody (GTX112760) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using mtTFA(GTX112760) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mtTFA protein stained by mtTFA antibody (GTX112760) diluted at 1:500.
Red: Mitotracker, a mitochondria marker, diluted at 1:2000.
Blue: Hoechst 33342 staining.

TFAM antibody detects TFAM protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TFAM stained by TFAM antibody (GTX112760) diluted at 1:1000.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

mtTFA antibody detects mtTFA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
mtTFA stained by mtTFA antibody (GTX112760) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Aging Cell in 2019. PMID: 31318148

The data was published in the 2021 in Physiol Rep. PMID: 33463897

The data was published in the 2021 in Physiol Rep. PMID: 33463897