Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with nNOS antibody (GTX133407) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

nNOS antibody detects nNOS protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: nNOS protein stained by nNOS antibody (GTX133407) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

nNOS antibody detects nNOS protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: nNOS stained by nNOS antibody (GTX133407) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

nNOS antibody detects nNOS protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
nNOS antibody (GTX133407) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

nNOS antibody detects nNOS protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
nNOS antibody (GTX133407) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min