Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas antibody [N2C2], Internal (GTX100605) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

p130Cas antibody [N2C2], Internal detects p130Cas protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
p130Cas antibody [N2C2], Internal (GTX100605) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

p130Cas antibody [N2C2], Internal detects p130Cas protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
p130Cas antibody [N2C2], Internal (GTX100605) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

p130Cas antibody [N2C2], Internal detects p130Cas protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: p130Cas protein stained by p130Cas antibody [N2C2], Internal (GTX100605) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

BCAR1 antibody [N2C2], Internal detects BCAR1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
BCAR1 antibody [N2C2], Internal (GTX100605) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

BCAR1 antibody [N2C2], Internal detects BCAR1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: BCAR1 protein stained by BCAR1 antibody [N2C2], Internal (GTX100605) diluted at 1:500.
Blue: Hoechst 33342 staining.

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX100605 diluted at 1:1000

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX100605 diluted at 1:1000