Untreated (–) and treated (+) NIH-3T3 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

p130Cas (phospho Tyr165) antibody detects p130Cas (phospho Tyr165) protein at cell junction by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: p130Cas (phospho Tyr165) protein stained by p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:500. (CIP: Calf-intestinal alkaline phosphatase)