Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr410) antibody [HL3401] (GTX641238) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr410) antibody [HL3401] (GTX641238) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.