Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

p21 Cip1 antibody [GT1032] immunoprecipitates CDKN1A protein in IP experiments.
IP samples: HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr
A. 30 μg HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr
B. Control with 4 μg of preimmune Mouse IgG
C. Immunoprecipitation of CDKN1A protein by 4 μg p21 Cip1 antibody [GT1032] (GTX629543)
15 % SDS-PAGE
The immunoprecipitated CDKN1A protein was detected by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:1000.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]

Untreated (–) and treated (+) HCT 116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HCT-116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
p21 Cip1 stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

p21 Cip1 antibody [GT1032] (GTX629543) detects p21 Cip1 by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: p21 Cip1 antibody [GT1032] (GTX629543) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDK4 protein stained by CDK4 antibody (GTX102993) diluted at 1:1000.
Red: p21 Cip1 protein stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500.
Blue: Hoechst 33342 staining.

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p21 Cip1 stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

The data was published in the journal PLoS One in 2015. PMID: 25961745

The data was published in the journal PLoS One in 2015. PMID: 25961745

The data was published in the journal PLoS One in 2015. PMID: 25961745