APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HCT116 (30 μM Cisplatin treatment for 24 hr) , HeLa
Predict Reactivity
Sheep, Bovine, Cat, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888123
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cyclin dependent kinase inhibitor 1A , CAP20 , CDKN1 , CIP1 , MDA-6 , P21 , SDI1 , WAF1 , p21CIP1
Cellular Localization
Cytoplasm , Nucleus
Background
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX629627 FACS Image
p21 Cip1 antibody [GT8611] (GTX629627) detects p21 Cip1 by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: p21 Cip1 antibody [GT8611] (GTX629627) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.
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GTX629627 WB Image
p21 Cip1 antibody [GT8611] detects p21 Cip1 protein by Western blot analysis. A. 30 μg HCT116 whole cell lysate/extract (untreated) B. 30 μg HCT116 whole cell lysate/extract (30 μM Cisplatin treatment for 24 hr) 15 % SDS-PAGE p21 Cip1 antibody [GT8611] (GTX629627) dilution: 1:1000
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GTX629627 ICC/IF Image
p21 Cip1 antibody [GT8611] detects p21 Cip1 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells treated with 30μM cisplatin for 24hr and were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 protein stained by p21 Cip1 antibody [GT8611] (GTX629627) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX629627 WB Image
Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT8611] (GTX629627) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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REFERENCE
REVIEW
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