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p21 Cip1 antibody

Orthogonal
Anti-p21 Cip1 antibody used in Western Blot (WB). GTX27960
Anti-p21 Cip1 antibody used in Western Blot (WB). GTX27960

Cat. No. GTX27960

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat
Package
1 ml ($399)
See all p21 Cip1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200
IHC-P Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX28015).

PROPERTIES

Form

Liquid

Buffer

PBS, 0.2% Gelatin

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide mapping to the carboxy terminus of human p21.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_367287

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase inhibitor 1A , CAP20 , CDKN1 , CIP1 , MDA-6 , P21 , SDI1 , WAF1 , p21CIP1

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-p21 Cip1 antibody used in Western Blot (WB). GTX27960

GTX27960 WB Image

Orthogonal

The data was published in the journal Biosci Rep in 2018. PMID: 29789400

Anti-p21 Cip1 antibody used in Western Blot (WB). GTX27960

GTX27960 WB Image

The data was published in the journal Int J Mol Sci in 2016. PMID: 27314341

REFERENCE

Application Reference

Ridwan SM et al. Cancer Immunol Immunother 2023; 72 (11) : 3851-3859 Radiation-induced dormancy of intracerebral melanoma: endotoxin inflammation leads to both shortened tumor dormancy and long-term survival with localized senescence.
Lin KI et al. Biosci Rep 2018; 38 (4) Carnosic acid impedes cell growth and enhances anticancer effects of carmustine and lomustine in melanoma.
Lee WJ et al. Int J Mol Sci 2016; 17 (6) The Effect of MCP-1/CCR2 on the Proliferation and Senescence of Epidermal Constituent Cells in Solar Lentigo.
Application : WB
Reactivity : Human
Chiu YS et al. Hepatology 2014; (Epub) IL-20 and IL-20R1 antibodies protect against liver fibrosis.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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Package List Price ($)
$ 399