Wild-type (WT) and CDKN1B knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody [HL2508] (GTX638869) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

p27 Kip1 antibody [HL2508] detects p27 Kip1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
p27 Kip1 stained by p27 Kip1 antibody [HL2508] (GTX638869) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

p27 Kip1 antibody [HL2508] detects p27 Kip1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
p27 Kip1 stained by p27 Kip1 antibody [HL2508] (GTX638869) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

p27 Kip1 antibody [HL2508] detects p27 Kip1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p27 Kip1 stained by p27 Kip1 antibody [HL2508] (GTX638869) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody [HL2508] (GTX638869) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody [HL2508] (GTX638869) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.