Immunohistochemical staining of human ulcerative colitis tissue using anti-p27 Kip1 antibody (GTX100446).

p27 Kip1 antibody detects p27 Kip1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p27 Kip1 protein stained by p27 Kip1 antibody (GTX100446) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody (GTX100446) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

p27 Kip1 antibody detects p27 Kip1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody (GTX100446) diluted at 1:1000.

Immunoprecipitation of p27 Kip1 protein from MCF-7 whole cell extracts using 5 μg of p27 Kip1 antibody (GTX100446).
Western blot analysis was performed using p27 Kip1 antibody (GTX100446).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Wild-type (WT) and p27 Kip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody (GTX100446) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

p27 Kip1 antibody detects p27 Kip1 protein at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: p27 Kip1 protein stained by p27 Kip1 antibody (GTX100446) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

The data was published in the journal EMBO J in 2018. PMID: 29084722

The data was published in the journal Int J Mol Sci in 2018. PMID: 29522490

The data was published in the journal Sci Rep in 2017. PMID: 28134285

The data was published in the journal Cell Death Dis in 2014.PMID: 25429617