Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

p53 antibody [HL2242] detects p53 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 stained by p53 antibody [HL2242] (GTX638291) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

p53 antibody [HL2242] detects p53 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
p53 stained by p53 antibody [HL2242] (GTX638291) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

p53 antibody [HL2242] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
p53 stained by p53 antibody [HL2242] (GTX638291) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.