p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.
Samples: HCT 116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 protein stained by p53 antibody [N1], N-term (GTX100629) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using p53(GTX100629) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term (GTX100629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using p53(GTX100629) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-p53 antibody (GTX100629). (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by GTX100629 diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit (GTX225856-01) was used in Western blot.

The data was published in the journal EMBO J in 2018. PMID: 29084722

The data was published in the 2022 in Toxics. PMID: 35622687