Sample (30 μg of whole cell lysate)
A: HCT116 cells with mock treatment for 24 hr
B: HCT116 cells with 30 μM cisplatin treatment for 24 hr
10% SDS PAGE
GTX70218 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using p53(GTX70218) antibody at 1:200 dilution.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [Pab240] (GTX70218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [Pab240] (GTX70218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.