ICC/IF analysis of A431 cells treated with 200 ng/ml of EGF for 10 minutes using GTX25231 p70 S6K (phospho Thr229) antibody. Panel e is untreated cell with nuclear localization. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

WB (peptide competition) analysis of Jurkat cells using GTX25231 p70 S6K (phospho Thr229) antibody prior incubated with the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.

The data was published in the journal Life Sci Alliance in 2019.PMID: 30923191

The data was published in the journal Life Sci Alliance in 2019.PMID: 30923191