Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with tPA antibody [HL2585] (GTX639036) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

tPA antibody [HL2585] detects tPA protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
tPA stained by tPA antibody [HL2585] (GTX639036) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

tPA antibody [HL2585] detects secreted tPA protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat thymus gland.
tPA stained by tPA antibody [HL2585] (GTX639036) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min