Wild-type (WT) and AKAP1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKAP1 antibody [C2C3], C-term (GTX102578) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)

A: IMR32

7.5% SDS PAGE

GTX102578 diluted at 1:1000