Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX106377 diluted at 1:3000

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TOM1L1 antibody [C3], C-term (GTX106377) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using TOM1L1(GTX106377) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.