Immunofluorescence analysis of methanol-fixed HepG2, using NDUFA12(GTX109567) antibody at 1:200 dilution.

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA12 antibody [N1C3] (GTX109567) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA12(GTX109567) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

NDUFA12 antibody [N1C3] detects NDUFA12 protein at mitochondria in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
NDUFA12 antibody [N1C3] (GTX109567) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA12 antibody [N1C3] (GTX109567) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.