NFIX antibody [HMV329] HistoMAX^TM

 

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Adrenal gland (Cortex) – Weak to moderate nuclear NFIX staining staining of adrenocortical cells

Adrenal gland (Medulla) – Distinct nuclear NFIX staining of few interspersed stromal (sustentacular cells_ endothelial_) cells while medullary cells remain negative

Aorta, media

Appendix, mucosa

Appendix, muscular wall – Distinct nuclear staining of smooth muscle cells and of other cell types

Bone marrow – Strong nuclear MRE11 staining of a rather small subset of cells (often arranged in groups)

Breast – Strong nuclear NFIX staining of a large subset of basal and luminal epithelial cells

Bronchus, mucosa – Distinct nuclear NFIX staining of most respiratory epithelial cells, but the staining intensity decreases markedly from the basal layer towards the more superficial cell layers

Cerebellum (white matter) – Weak to moderate nuclear NFIX staining of glial cells

Cerebellum, cortex (molecular layer, Purkinje cell layer, granule cell layer) – Weak nuclear NFIX staining of glial and granular cells. Lack of staining NFIX in Purkinje cell

Cerebrum, grey matter – Strong nuclear NFIX staining of glial cells. Weak or absent staining in neurons

Cerebrum, white matter – Strong nuclear NFIX staining of glial cells

Colon descendens, mucosa – Moderate nuclear NFIX staining of epithelial cells

Colon descendens, muscular wall – Distinct nuclear staining of smooth muscle cells and of other cell types

Duodenum, Brunner gland – Distinct nuclear MRE11 staining of all epithelial cells, including Brunner glands

Duodenum, mucosa – Nuclear NFIX staining is moderate in crypt bases and weak-faint or even absent in the surface epithelium

Epididymis (Caput and cauda) – Strong nuclear NFIX staining of epithelial cells in the cauda. In the caput, the NFIX staining is only faint in chief cells but strong in basal cells

Epididymis (Cauda) – Strong nuclear NFIX staining of epithelial cells

Esophagus, squamous epithelium – Significant decrease of nuclear NFIX staining of squamous epithelium from the basal-suprabasal to the superficial cell layers

Fallopian tube, mucosa – Strong nuclear NFIX staining of all cells

Fat

Gallbladder, epithelium – Nuclear NFIX staining is weak to moderate in epithelial cells and stronger in stromal cells

Heart muscle – Distinct nuclear staining of heart muscle cells and of other cell types

Ileum, mucosa – Nuclear NFIX staining is moderate in crypt bases and weak-faint or even absent in the surface epithelium

Kidney, cortex – Nuclear NFIX staining is generally low in all cell types and ranges from absent to faint-weak

Kidney, medulla – Nuclear NFIX staining is generally low in all cell types and ranges from absent to faint-weak

Kidney, pelvis, urothelium – Distinct nuclear NFIX staining of urothelial cells although there is a significant decrease of NFIX staining intensity from the basal to the superficial cell layers. Umbrella cells are largely NFIX negative

Liver – Nuclear NFIX staining is largely absent in hepatocytes. NFIX staining is only seen in stromal cells

Lung – Strong nuclear NFIX staining of all cell types (except macrophages- these remain NFIX negative)

Ovary, corpus luteum – Strong nuclear NFIX staining of stromal cells. Corpus luteum cells are NFIX negative

Ovary, stroma – Strong nuclear NFIX staining of stromal cells

Pancreas – Strong nuclear NFIX staining of epithelial cells of excretory ducts and of intercalated ducts. NFIX staining is markedly weaker in acinar cells

Pancreas – Strong nuclear NFIX staining of stromal and intercalated duct cells. NFIX staining is faint or absent in acinar cells

Parathyroid gland – Moderate nuclear NFIX staining of all epithelial cells

Parotid gland

Pituitary gland, anterior lobe – Only few cells show a moderate to strong nuclear NFIX staining

Pituitary gland, posterior lobe – Strong nuclear NFIX staining of all pituicytes

Placenta (amnion)

Placenta (chorion)

Placenta, early – Strong nuclear MRE11 staining of stromal cells while trophoblast cells remain negative

Placenta, mature – Strong nuclear NFIX staining of stromal cells while trophoblast cells remain negative

Prostate – Strong nuclear NFIX staining of stromal and all epithelial cells

Rectum, mucosa – Weak to moderate nuclear NFIX staining of epithelial cells. Slight but significant decrease of NFIX staining intensity from the crypt base to the superficial epithelial cell layers

Seminal vesicle – Strong nuclear NFIX staining of stromal and basal epithelial cells. NFIX staining is weak or absent in most luminal epithelial cells

Sinus paranasales – Distinct nuclear NFIX staining of respiratory epithelial cells but the staining intensity decreases from the basal layer towards the more superficial cell layers

Skeletal muscle – Distinct nuclear staining of skeletal muscle cells and of other cell types

Skin – Strong nuclear NFIX staining of squamous epithelial cells

Skin, hairfollicel and sebaceous glands – Weak to moderate nuclear NFIX staining of sebaceous gland cells. The staining intensity decreases towards the centre of the glands

Spleen – Distinct nuclear NFIX staining of some stromal and endotheliel cells. Lymphocytes are NFIX negative

Stomach, antrum – Moderate to strong nuclear NFIX staining of most epithelial cells but few glandular cells do only show faint or even absent NFIX staining

Stomach, muscular – Distinct nuclear staining of smooth muscle cells and of other cell types

Testis – Distinct nuclear NFIX staining of intertubular cells (including Leydig cells). Complete absence of NFIX staining in tubular cells (Sertoli cells, germ cells)

Thymus – Lymphocytes are NFIX negative. Distinct nuclear NFIX staining of epithelial cells. Maturing squamous epithelial cells of corpuscles of Hassall's stain weakly or remain negative

Thymus – Lymphocytes are NFIX negative. Distinct nuclear NFIX staining of thymic epithelial cells

Thyroid gland – Strong nuclear MRE11 staining of follicular cells

Tonsil – Distinct nuclear NFIX staining of a subset of squamous epithelial cells. Decreasing staining intensity from basal to the superficial cell layers. Some stromal cells and endothelial cells are strongly positive. Lymphocytes are largely negativ

Tonsil, surface epithelium – Significant decrease of nuclear NFIX staining of squamous epithelium from the basal-suprabasal to the superficial cell layers

Urinary bladder, muscular wall – Distinct nuclear staining of smooth muscle cells and of other cell types

Urinary bladder, urothelium – Distinct nuclear NFIX staining of urothelial cells although there is a significant decrease of NFIX staining intensity from the basal to the superficial cell layers. Umbrella cells are largely NFIX negative

Uterus, endocervix – Moderate nuclear NFIX staining of glandular cells. Strong nuclear NFIX staining of stromal cells

Uterus, endometrium (pregnancy)

Uterus, endometrium (proliferation) – Strong nuclear NFIX staining of stromal cells while endometrium cells remain NFIX negative

Uterus, endometrium (secretion) – Strong nuclear NFIX staining of stromal cells while endometrium cells remain NFIX negative

Uterus, myometrium – Distinct nuclear staining of smooth muscle cells of the myometrium

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