Human Respiratory Syncytial Virus (RSV) is an underdiagnosed, often lethal respiratory pathogen affecting infants and children as well as the elderly, immunocompromised, and long-term care facility populations. Infection, which occurs through aerosolized droplets and close contact, is essentially universal within the first two years of life and reinfection is common in both children and adults. Though often thought to be clinically less severe than influenza, RSV is linked to perhaps 250,000 adult, and 60,000 pediatric, deaths per year.

RSV is a filamentous/spherical, enveloped, negative-sense, single-stranded RNA virus of the Pneumoviridae family. Its 15.2 kb genome encodes eleven proteins from ten genes (1). Though the two major RSV antigenic subgroups, designated “A” and “B”, display differences in multiple proteins, the primary antigenic determinant is the “G” attachment glycoprotein. Interestingly, it is the “prefusion” form of the “F” fusion protein that has proven to be the most successful immunogenic RSV factor (via its “theta” site) in terms of eliciting neutralizing antibodies and generating promising vaccine trials against both RSV Type A and Type B (2). Several monoclonal antibody therapies directed against prefusion F are in development to augment the one available RSV monoclonal antibody therapy (palivizumab) that is indicated for high-risk infants, though it targets postfusion F and has both cost and administration issues. 

There are a number of approaches to diagnose RSV. However, the most reliable method presently is RT-PCR performed on nasopharyngeal swab samples. Antigen testing for rapid point-of-care diagnosis would greatly facilitate diagnosis as well as potentially more effective early intervention. For this reason, and to support additional research into basic RSV biology, GeneTex is developing recombinant rabbit monoclonal antibodies against various RSV proteins, focusing first on the RSV nucleoprotein (indicated as NP below). The initial series of NP antibodies have superior binding affinity and are validated for ELISA, sandwich ELISA (sELISA), and lateral flow assay (LFA) (see below and individual datasheets). In addition, several of them perform well in western blot, immunoprecipitation, and immunocytochemistry.

Please see the data shown below and contact us at www.genetex.com with any questions.

 

Recommended RSV Antibody Pairs for Lateral Flow Assay

Detection Antibody GTX636649 [HL1247] GTX636650 [HL1248] GTX636648 [HL1246] GTX636650 [HL1248] GTX636648 [HL1246] GTX636649 [HL1247] Capture Antibody GTX636648 [HL1246] GTX636649 [HL1247] GTX636650 [HL1248] Sample Respiratory Syncytial Virus type A Nucleoprotein (GTX136751-pro)

 

Comparison of GeneTex’s Recombinant RSV Antibodies with other Market-leading Clones

Indirect ELISA assays exhibit the comparative sensitivities of recombinant RSV Nucleoprotein (NP) antibodies GTX636648, GTX636649, and GTX636650 against (A) RSV A NP or (B) RSV B NP, compared to clones from two competitors. 

Recommended RSV Antibody Pairs for Sandwich ELISA

Capture Antibody GTX636649 [HL1247]   Capture Antibody GTX636650 [HL1248]   Detection Antibody GTX636648 [HL1246]   Detection Antibody GTX636648 [HL1246]

 

Featured Recombinant RSV Antibodies

  

Cat. No. Product Name Clonality Applications GTX636647 Respiratory Syncytial virus Nucleoprotein antibody [HL1245] Rec Rb mAb WB, ELISA, Sandwich ELISA GTX636648 Respiratory Syncytial Virus Nucleoprotein antibody [HL1246] Rec Rb mAb WB, ICC/IF, IP, ELISA, Lateral Flow, Sandwich ELISA GTX636649 Respiratory Syncytial Virus Nucleoprotein antibody [HL1247] Rec Rb mAb WB, IP, ELISA, Lateral Flow, Sandwich ELISA   GTX636650 Respiratory Syncytial Virus Nucleoprotein antibody [HL1248] Rec Rb mAb WB, ICC/IF, IP, ELISA, Lateral Flow, Sandwich ELISA GTX636705 Respiratory Syncytial Virus Nucleoprotein antibody [HL1291] Rec Rb mAb WB, ELISA, Sandwich ELISA GTX636709 Respiratory Syncytial Virus Nucleoprotein antibody [HL1295] Rec Rb mAb WB, ICC/IF, ELISA, Sandwich ELISA GTX636710 Respiratory Syncytial Virus Nucleoprotein antibody [HL1296] Rec Rb mAb WB, ICC/IF, ELISA, Sandwich ELISA GTX636711 Respiratory Syncytial Virus type A Nucleoprotein antibody [HL1297] Rec Rb mAb WB, ICC/IF, IP, ELISA, Sandwich ELISA GTX637381 Respiratory Syncytial virus type B G protein antibody [HL1733] Rec Rb mAb WB, ICC/IF

 

Superior Binding Affinities of Recombinant RSV Antibodies

  

Cat. No. Clone EC50 RSV A NP (GTX136751-pro) EC50 RSV B NP (GTX136931-pro) GTX636648 HL1246 13.6 pM     15.4 pM GTX636649 HL1247   3.8 pM     15.9 pM    GTX636650 HL1248   8.9 pM     10.1 pM GTX636705 HL1291 10.7 pM     19.0 pM GTX636709 HL1295 19.8 pM   105.4 pM GTX636710 HL1296 29.0 pM   170.8 pM GTX636711* HL1297 16.7 pM 2854.8 pM

* GTX636711 is specific for RSV A NP.

RSV Nucleoprotein Recombinant Proteins

 

Cat. No. Product Name Expression System Applications GTX136751-pro Respiratory Syncytial Virus type A Nucleoprotein, DDDDK tag HEK293 WB, ELISA, Lateral Flow, Sandwich ELISA GTX136931-pro Respiratory Syncytial Virus type B Nucleoprotein, DDDDK tag HEK293 WB, ELISA, Sandwich ELISA

 

 

Highlighted Products

 

Respiratory Syncytial Virus type A Nucleoprotein, DDDDK tag (GTX136751-pro) Respiratory Syncytial Virus type B Nucleoprotein, DDDDK tag (GTX136931-pro)

Respiratory Syncytial Virus Nucleoprotein antibody [HL1248] (GTX636650)

Respiratory Syncytial Virus type A Nucleoprotein antibody [HL1297] (GTX636711)

 

References:
(1) Nat Rev Microbiol. 2019 Apr;17(4):233-245.
(2) Pathogens. 2022 Nov 11;11(11):1324.