4E-BP1 antibody [N1C3]

Name: 4E-BP1 antibody [N1C3]
Brand: GeneTex
SKU: GTX109162
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-4E-BP1 antibody [N1C3] used in Immunoprecipitation (IP). GTX109162

Anti-4E-BP1 antibody [N1C3] used in Western Blot (WB). GTX109162

Anti-4E-BP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109162

Cat. No. GTX109162

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IP
Reactivity	Human, Rat
Package	100 μl ($399), 25 μl ($169)

See all 4E-BP1 products

View product citations for antibody GTX109162 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

H1299 , HCT116 , HeLa

Predict Reactivity

Mouse, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human 4E-BP1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163771

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4E binding protein 1 , 4E-BP1 , 4EBP1 , BP-1 , PHAS-I

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq]

Database

Gene ID: 1978 EIF4EBP1
UniProt: Q13541 EIF4EBP1

Research Area

DATA IMAGES

GTX109162 IP Image

Immunoprecipitation of 4E-BP1 protein from HCT116 whole cell extracts using 5 μg of 4E-BP1 antibody [N1C3] (GTX109162) or 4E-BP1 antibody [N1C3-2] (GTX116315).
Western blot analysis was performed using 4E-BP1 antibody [N1C3] (GTX109162).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX109162 WB Image

Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3] (GTX109162) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109162 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using EIF4EBP1(GTX109162) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109162 WB Image

4E-BP1 antibody detects 4E-BP1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody (GTX109162) diluted at a dilution of 1:2000.

REFERENCE

Application Reference

Yanqi Xie et al. Mol Cancer Ther 2021; 20 (10) : 1893-1903 Potent Synergistic Effect on C-Myc-Driven Colorectal Cancers Using a Novel Indole-Substituted Quinoline with a Plk1 Inhibitor.

Application : WB

Reactivity : Human

Wu YY et al. J Exp Clin Cancer Res 2019; 38 (1) : 282 The dual PI3K/mTOR inhibitor BEZ235 restricts the growth of lung cancer tumors regardless of EGFR status, as a potent accompanist in combined therapeutic regimens.

Application : WB

Reactivity : Human

Liu YH et al. Phytomedicine 2018; 46 : 184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.

Application : WB

Reactivity : Human

Yeh YH et al. J Exp Clin Cancer Res 2018; 37 (1) : 70 inflammatory * activates hif-1α-mediated epithelial-to-mesenchymal transition via pi3k/akt/mtor pathway.

Application : WB

Reactivity : Human

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